-
Product Name
TOP1 antibody
- Documents
-
Description
TOP1 Rabbit Polyclonal antibody. Positive WB detected in L02 cells, HeLa cells. Positive IP detected in L02 cells. Positive IHC detected in human cervical cancer tissue. Observed molecular weight by Western-blot: 70 kDa
-
Tested applications
ELISA, WB, IHC, IP
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
DNA topoisomerase 1 antibody; DNA topoisomerase I antibody; TOP1 antibody; TOPI antibody; topoisomerase (DNA) I antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_003286). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
-
Validations
L02 cells were subjected to SDS PAGE followed by western blot with Catalog No:116244(TOP1 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:116244(TOP1 antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-TOP1 (IP:Catalog No:116244, 4ug; Detection:Catalog No:116244 1:500) with L02 cells lysate 1800ug.
-
Background
TOP1 belongs to the eukaryotic type I topoisomerase family. The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. The antibody is specific to TOP1.
-
References
- Wu J, Yang C, Guo C. SZC015, a synthetic oleanolic acid derivative, induces both apoptosis and autophagy in MCF-7 breast cancer cells. Chemico-biological interactions. 244:94-104. 2016.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"