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Product Name
Tom40 antibody
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Description
Tom40 Rabbit Polyclonal antibody. Positive IP detected in mouse liver tissue. Positive WB detected in human brain tissue, A431 cells, HEK-293 cells, HepG2 cells, human liver tissue. Positive IHC detected in human liver tissue, human brain tissue, human heart tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human testis tissue. Positive IF detected in HepG2 cells, HeLa cells. Positive FC detected in HepG2 cells. Observed molecular weight by Western-blot: 38 kDa
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Tested applications
ELISA, WB, IF, IP, IHC, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
C19orf1 antibody; D19S1177E antibody; p38.5 antibody; PER EC1 antibody; PEREC1 antibody; Protein Haymaker antibody; TOM40 antibody; TOMM40 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Tom40 recombinant protein (Accession Number: NM_001128916). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:116173(TOMM40 antibody) at dilution of 1:600
Immunofluorescent analysis of HepG2 cells using Catalog No:116173(TOMM40 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
Immunohistochemistry of paraffin-embedded human liver tissue slide using Catalog No:116173(TOMM40 Antibody) at dilution of 1:200 (under 40x lens).
Immunohistochemistry of paraffin-embedded human liver tissue slide using Catalog No:116173(TOMM40 Antibody) at dilution of 1:200 (under 10x lens).
IP Result of anti-TOMM40 (IP:Catalog No:116173, 4ug; Detection:Catalog No:116173 1:1000) with mouse liver tissue lysate 4000ug.
1X10^6 HepG2 cells were stained with .2ug TOMM40 antibody (Catalog No:116173, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
TOMM40 represents translocase of outer mitochondrial membrane 40 homolog (yeast)
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References
- Ding C, Wu Z, Huang L. Mitofilin and CHCHD6 physically interact with Sam50 to sustain cristae structure. Scientific reports. 5:16064. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"