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Product Name
TOM20 antibody
- Documents
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Description
TOM20 Rabbit Polyclonal antibody. Positive FC detected in HeLa cells. Positive IHC detected in human gliomas tissue. Positive IP detected in HEK-293 cells. Positive WB detected in mouse brain tissue, HEK-293 cells, HeLa cells, mouse liver tissue. Observed molecular weight by Western-blot: 16kd
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Tested applications
ELISA, IHC, WB, IP, FC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
KIAA0016 antibody; MAS20 antibody; MOM19 antibody; TOM20 antibody; TOMM20 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TOM20 recombinant protein (Accession Number: NM_014765). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:1000-1:10000
IHC: 1:20-1:200
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Validations
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:116171(TOM20 antibody) at dilution of 1:100 (under 10x lens)
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:116171(TOM20 Antibody) at dilution of 1:2000
IP Result of anti-TOM20 (IP:Catalog No:116171, 3ug; Detection:Catalog No:116171 1:2000) with HEK-293 cells lysate 2400ug.
1X10^6 HeLa cells were stained with 0.2ug TOM20 antibody (Catalog No:116171, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
TOMM20, also named as KIAA0016, belongs to the Tom20 family. It is a central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22, TOMM20 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. TOMM20 is characterized as major docking receptors to mediate the recognition by different mechanisms.
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References
- Wei B, Cui Y, Huang Y. Tom70 mediates Sendai virus-induced apoptosis on mitochondria. Journal of virology. 89(7):3804-18. 2015.
- Fiesel FC, Moussaud-Lamodière EL, Ando M, Springer W. A specific subset of E2 ubiquitin-conjugating enzymes regulate Parkin activation and mitophagy differently. Journal of cell science. 127(Pt 16):3488-504. 2014.
- Zhang X, Zuo X, Yang B. MicroRNA directly enhances mitochondrial translation during muscle differentiation. Cell. 158(3):607-19. 2014.
- Caulfield TR, Fiesel FC, Moussaud-Lamodière EL, Dourado DF, Flores SC, Springer W. Phosphorylation by PINK1 releases the UBL domain and initializes the conformational opening of the E3 ubiquitin ligase Parkin. PLoS computational biology. 10(11):e1003935. 2014.
- Fiesel FC, Caulfield TR, Moussaud-Lamodière EL. Structural and Functional Impact of Parkinson Disease-Associated Mutations in the E3 Ubiquitin Ligase Parkin. Human mutation. 36(8):774-86. 2015.
- Gao J, Qin S, Jiang C. Parkin-induced ubiquitination of Mff promotes its association with p62/SQSTM1 during mitochondrial depolarization. Acta biochimica et biophysica Sinica. 47(7):522-9. 2015.
- Murakawa T, Yamaguchi O, Hashimoto A. Bcl-2-like protein 13 is a mammalian Atg32 homologue that mediates mitophagy and mitochondrial fragmentation. Nature communications. 6:7527. 2015.
- Deng J, Yang M, Chen Y. FUS Interacts with HSP60 to Promote Mitochondrial Damage. PLoS genetics. 11(9):e1005357. 2015.
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