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Product Name
TOE1 antibody
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Description
TOE1 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human colon cancer tissue. Positive WB detected in HeLa cells, mouse thymus tissue. Observed molecular weight by Western-blot: 57kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ13949 antibody; Target of EGR1 protein 1 antibody; TOE1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TOE1 recombinant protein (Accession Number: NM_025077). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:116166(TOE1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:116166(TOE1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:116166(TOE1 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using TOE1 antibody Catalog No:116166 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
TOE1 represents target of EGR1, member 1 (nuclear)
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"