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Product Name
TNRC6A Polyclonal Antibody
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Description
Polyclonal antibody to TNRC6A
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
TNRC6A antibody; CAGH26 antibody; GW1 antibody; GW182 antibody; TNRC6 antibody; trinucleotide repeat containing 6A antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 950-1050 of human TNRC6A (NP_055309.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
IF 1:50 - 1:100 -
Validations
Western blot - TNRC6A Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using TNRC6A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - TNRC6A Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using TNRC6A antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Plays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA-dependent repression of translation and for siRNA-dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins. As a scaffolding protein, associates with argonaute proteins bound to partially complementary mRNAs, and can simultaneously recruit CCR4-NOT and PAN deadenylase complexes.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"