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Product Name
TNFRSF25 Polyclonal Antibody
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Description
Polyclonal antibody to TNFRSF25
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
TNFRSF25 antibody; APO-3 antibody; DDR3 antibody; DR3 antibody; LARD antibody; TNFRSF12 antibody; TR3 antibody; TRAMP antibody; WSL-1 antibody; WSL-LR antibody; TNF receptor superfamily member 25 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 240-426 of human TNFRSF25 (NP_683866.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - TNFRSF25 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using TNFRSF25 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.
Immunofluorescence - TNFRSF25 Polyclonal Antibody
Immunofluorescence analysis of human colon carcinoma cells using TNFRSF25 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - TNFRSF25 Polyclonal Antibody
Immunofluorescence analysis of rat breast cells using TNFRSF25 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Receptor for TNFSF12/APO3L/TWEAK. Interacts directly with the adapter TRADD. Mediates activation of NF-kappa-B and induces apoptosis. May play a role in regulating lymphocyte homeostasis.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"