Product Name

TNFR1 antibody

Documents

Description

TNFR1 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HL-60 cells, human brain tissue, NIH/3T3 cells, Raji cells. Positive FC detected in Raji cells. Observed molecular weight by Western-blot: 40-45 kDa,60 kDa

Tested applications

ELISA, WB, FC

Species reactivity

Human, Mouse; other species not tested.

Alternative names

CD120a antibody; FPF antibody; p55 antibody; p55 R antibody; p60 antibody; TBP1 antibody; TBPI antibody; TNF R antibody; TNF R I antibody; TNF R1 antibody; TNF R55 antibody; TNF Receptor 1 antibody; TNF Receptor I antibody; TNF RI antibody; TNFAR antibody; TNFR I antibody; TNFR1 antibody; TNFR55 antibody; TNFR60 antibody; TNFRSF1A antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of TNFR1 recombinant protein (Accession Number: NM_001065). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

Validations

HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:116289(TNFR1 antibody) at dilution of 1:500

1X10^6 Raji cells were stained with 0.2ug TNFR1 antibody (Catalog No:116289, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Background

Tumor necrosis factor (TNF) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNF exists in soluble and membrane-bound forms. TNF signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a) and TNFR2 (TNFRSF1B, CD120b). Whereas TNFR1 is widely expressed, expression of TNFR2 is limited to cells of the immune system, endothelial cells, and nerve cells (PMID: 22053109). TNFR1, which contains a death domain (DD) within its intracytoplasmic region, is thought to be the key receptor for TNF signaling (PMID: 16407280). This receptor can activate NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor.

References

Zhang C, Wang C, Tang S. TNFR1/TNF-α and mitochondria interrelated signaling pathway mediates quinocetone-induced apoptosis in HepG2 cells. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 62:825-38. 2013.
Kallenborn-Gerhardt W, Hohmann SW, Syhr KM. Nox2-dependent signaling between macrophages and sensory neurons contributes to neuropathic pain hypersensitivity. Pain. 155(10):2161-70. 2014.
Yang T, Shi R, Chang L. Huachansu suppresses human bladder cancer cell growth through the Fas/Fasl and TNF- alpha/TNFR1 pathway in vitro and in vivo. Journal of experimental & clinical cancer research : CR. 34:21. 2015.
Murtishaw AS, Heaney CF, Bolton MM, Sabbagh JJ, Langhardt MA, Kinney JW. Effect of acute lipopolysaccharide-induced inflammation in intracerebroventricular-streptozotocin injected rats. Neuropharmacology. 101:110-22. 2016.
Nie D, Zhang D, Dai J. Nicotine Induced Murine Spermatozoa Apoptosis via Up-Regulation of Deubiquitinated RIP1 by Trim27 Promoter Hypomethylation. Biology of reproduction. 94(2):31. 2016.

TNFR1 antibody

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