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Product Name
TNFAIP1 antibody
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Description
TNFAIP1 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, L02 cells, Transfected HEK-293 cells. Positive IF detected in HeLa cells. Positive IHC detected in human placenta tissue, human brain tissue. Observed molecular weight by Western-blot: 36 kDa
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Tested applications
ELISA, IHC, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
B12 antibody; B61 antibody; BACURD2 antibody; EDP1 antibody; hBACURD2 antibody; Protein B12 antibody; TNFAIP1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TNFAIP1 recombinant protein (Accession Number: NM_021137). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:116285(TNFAIP1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human placenta using Catalog No:116285(TNFAIP1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human placenta using Catalog No:116285(TNFAIP1 antibody) at dilution of 1:50 (under 40x lens)
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:116285(TNFAIP1 Antibody) at dilution of 1:1000
Immunofluorescent analysis of HeLa cells using Catalog No:116285(TNFAIP1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
TNFAIP1 was identified as a human gene activated transcriptionally by tumour necrosis factor alpha in endothelial cells. It is encoded by a 3.5-kilobase transcript and its expression is found to be developmentally regulated in a tissue-specific manner. The open reading frame predicts a 316-amino acid sequence rich in charged residues, particularly at the carboxyl terminus.
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References
- Zhang X, Li X, Tan Z. MicroRNA-373 is upregulated and targets TNFAIP1 in human gastric cancer, contributing to tumorigenesis. Oncology letters. 6(5):1427-1434. 2013.
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