Cynomolgus BLyS/TNFSF13B (Fc Tag) recombinant protein

Cynomolgus BLyS/TNFSF13B (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

B lymphocyte stimulator (BLyS), also known as TNFSF13B, CD257 and BAFF, is single-pass type II membrane protein, which belongs to the tumor necrosis factor family. BAFF is abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. BAFF is a cytokine and serves as a ligand for receptors TNFRSF13B (TACI), TNFRSF17 (BCMA), and TNFRSF13C (BAFFR). These receptors is a prominent factor in B cell differentiation, homeostasis, and selection. BLyS levels affect survival signals and selective apoptosis of autoantibody-producing B cells. Thus, it acts as a potent B cell activator and has been shown to play an important role in the proliferation and differentiation of B cells. Overexpression of BLyS in mice can lead to clinical and serological features of systemic lupus erythematosus (SLE) and Sjögren's syndrome (SS). BLyS as an attractive therapeutic target in human rheumatic diseases. The ability of BLyS to regulate both the size and repertoire of the peripheral B cell compartment raises the possibility that BLyS and antagonists thereof may form the basis of a therapeutic trichotomy. As an agonist, BLyS protein may enhance humoral immunity in congenital or acquired immunodeficiencies such as those resulting from viral infection or cancer therapy.

TNF-and APOL-related leukocyte expressed ligand 1

TALL-1; MGC124061; CD257; MGC124060; BAFF; DTL; TALL1; THANK; TNFSF13B; BLYS; TNFSF20; D8ERTD387E; ZTNF4

F7H548

TNFSF13B; EGK_09455

Human Cells

A DNA sequence encoding the cynomolgus TNFSF13B (F7H548) (Ala134-Leu285) was expressed with the Fc region of human IgG1 at the C-terminus.

Cynomolgus

The recombinant cynomolgus TNFSF13B is a disulfide-linked homodimer. The reduced monomer comprises 412 amino acids and has a calculated molecular mass of 45.5 KDa.The apparent molecular mass of the protein is approximately 47 KDa respectively in SDS-PAGE.

> 95 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.
Immobilized Cynomolgus Fc-TNFSF13B at 10 μg/ml (100 μl/well) can bind biotinylated Cynomolgus TNFRSF17-Fc (Cat:500916), the EC₅₀ of biotinylated Cynomolgus TNFRSF17-Fc (Cat:500916) is 4.5-10.5 ng/ml.