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Product Name
TLR1 Polyclonal Antibody
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Description
Polyclonal antibody to TLR1
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
TLR1 antibody; CD281 antibody; TIL antibody; TIL. LPRS5 antibody; rsc786 antibody; TIL.LPRS5 antibody; toll-like receptor 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-290 of human TLR1 (NP_003254.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - TLR1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using TLR1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.
Immunofluorescence - TLR1 Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using TLR1 antibody . Blue: DAPI for nuclear staining.
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Background
Participates in the innate immune response to microbial agents. Specifically recognizes diacylated and triacylated lipopeptides. Cooperates with TLR2 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Forms the activation cluster TLR2:TLR1:CD14 in response to triacylated lipopeptides, this cluster triggers signaling from the cell surface and subsequently is targeted to the Golgi in a lipid-raft dependent pathway. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"