-
Product Name
TK1 antibody
- Documents
-
Description
TK1 Rabbit Polyclonal antibody. Positive IP detected in HEK-293 cells. Positive WB detected in HEK-293 cells, MCF7 cells. Positive IF detected in Hela cells. Positive IHC detected in human breast cancer tissue. Observed molecular weight by Western-blot: 25 kDa
-
Tested applications
ELISA, WB, IF, IHC, IP
-
Species reactivity
Human, Mouse, Rat; other species not tested.
-
Alternative names
thymidine kinase 1 antibody; soluble antibody; Thymidine kinase antibody; cytosolic antibody; TK1 antibody; TK2 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of TK1 recombinant protein (Accession Number: NM_003258). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
-
Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:116067(TK1 antibody) at dilution of 1:300
Immunofluorescent analysis of Hela cells, using TK1 antibody Catalog No:116067 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-TK1 (IP:Catalog No:116067, 4ug; Detection:Catalog No:116067 1:300) with HEK-293 cells lysate 2000ug.
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:116067(TK1 Antibody) at dilution of 1:50 (under 40x lens)
-
Background
TK1(Thymidine kinase, cytosolic) belongs to the thymidine kinase family and is a regulatory enzyme that can interchange between its dimeric and tetrameric forms, which have low and high catalytic efficiencies, respectively. TK1 is involved in DNA synthesis and an important proliferation marker(PMID:21178264).
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"