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Product Name
TIP47 antibody
- Documents
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Description
TIP47 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HEK-293 cells, Jurkat cells. Positive IP detected in HeLa cells. Positive IF detected in MEF cells. Positive FC detected in HeLa cells. Observed molecular weight by Western-blot: 47 kDa
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Tested applications
ELISA, WB, IF, IP, FC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
M6PRBP1 antibody; Perilipin 3 antibody; Placental protein 17 antibody; PLIN3 antibody; TIP47 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TIP47 recombinant protein (Accession Number: NM_005817). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IF: 1:20-1:200
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Validations
IF result of anti-TIP47 (Catalog No:116063,1:500) with MEF cell by Dr.Hector Alex Saka.
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:116063(M6PRBP1 antibody) at dilution of 1:1000
IP Result of anti-M6PRBP1 (IP:Catalog No:116063, 3ug; Detection:Catalog No:116063 1:1000) with HeLa cells lysate 1000ug.
1X10^6 HeLa cells were stained with 0.2ug M6PRBP1 antibody (Catalog No:116063, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
Mannose 6-phosphate receptors (M6PRs) transport newly synthesized lysosomal hydrolases from the Golgi to prelysosomes and then return to the Golgi for another round of transport. M6PRBP1 (mannose-6-phosphate receptor binding protein 1), also known as TIP47, PLIN3 or PP17, interacts with the cytoplasmic domains of both cation-independent and cation-dependent M6PRs, and is required for endosome-to-Golgi transport. In addition to M6PR recycling, M6PRBP1 plays a role in lipid droplet biogenesis, and is also implicated in rhodopsin photobleaching and viral infection. M6PRBP1 has been found to be expressed in a variety of human tissues (including colon, liver and lung parenchyme, mammary gland, and skin) and is overexpressed in certain cancer cell lines. It binds to lipid droplets and also occurs in cytosol and on endosomal membranes.
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References
- Eastman SW, Yassaee M, Bieniasz PD. A role for ubiquitin ligases and Spartin/SPG20 in lipid droplet turnover. The Journal of cell biology. 184(6):881-94. 2009.
- Haldar AK, Saka HA, Piro AS. IRG and GBP host resistance factors target aberrant, "non-self" vacuoles characterized by the missing of "self" IRGM proteins. PLoS pathogens. 9(6):e1003414. 2013.
- Gawlik K, Baugh J, Chatterji U, Lim PJ, Bobardt MD, Gallay PA. HCV core residues critical for infectivity are also involved in core-NS5A complex formation. PloS one. 9(2):e88866. 2014.
- Langhi C, Marquart TJ, Allen RM, Baldán A. Perilipin-5 is regulated by statins and controls triglyceride contents in the hepatocyte. Journal of hepatology. 61(2):358-65. 2014.
- Langhi C, Baldán Á. CIDEC/FSP27 is regulated by peroxisome proliferator-activated receptor alpha and plays a critical role in fasting- and diet-induced hepatosteatosis. Hepatology (Baltimore, Md.). 61(4):1227-38. 2015.
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