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Product Name
TIMP3 Polyclonal Antibody
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Description
Polyclonal antibody to TIMP3
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Tested applications
WB, FC
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Species reactivity
Human, Rat
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Alternative names
TIMP3 antibody; HSMRK222 antibody; K222 antibody; K222TA2 antibody; SFD antibody; metalloproteinase inhibitor 3 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human TIMP3 (NP_000353.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
FC 1:50 - 1:200 -
Validations
Western blot - TIMP3 Polyclonal Antibody
Western blot analysis of extracts of 293T cells, using TIMP3 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.
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Background
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"