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Product Name
TIMP-2 antibody
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Description
TIMP-2 Rabbit Polyclonal antibody. Positive WB detected in Transfected HEK-293 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human placenta tissue, human brain tissue, human cervical cancer tissue, human heart tissue, human kidney tissue, human lung tissue, human skin tissue, human spleen tissue, human testis tissue.
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Tested applications
ELISA, IHC, IF, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CSC 21K antibody; Metalloproteinase inhibitor 2 antibody; TIMP 2 antibody; TIMP2 antibody; TIMP-2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TIMP-2 recombinant protein (Accession Number: NM_003255). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Immunohistochemical of paraffin-embedded human placenta using Catalog No:116140(TIMP2 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human placenta using Catalog No:116140(TIMP2 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using TIMP2 antibody Catalog No:116140 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:116140(TIMP2 Antibody) at dilution of 1:1000
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Background
TIMP2 represents TIMP metallopeptidase inhibitor 2
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References
- Lin JJ, Su JH, Tsai CC, Chen YJ, Liao MH, Wu YJ. 11-epi-Sinulariolide acetate reduces cell migration and invasion of human hepatocellular carcinoma by reducing the activation of ERK1/2, p38MAPK and FAK/PI3K/AKT/mTOR signaling pathways. Marine drugs. 12(9):4783-98. 2014.
- Xie Y, Cui D, Sui L. Induction of forkhead box M1 (FoxM1) by EGF through ERK signaling pathway promotes trophoblast cell invasion. Cell and tissue research. 362(2):421-30. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"