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Product Name
TIMM17A Polyclonal Antibody
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Description
Polyclonal antibody to TIMM17A
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Tested applications
WB, IF
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Species reactivity
Human, Mouse
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Alternative names
TIMM17A antibody; TIM17 antibody; TIM17A antibody; translocase of inner mitochondrial membrane 17A antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-171 of human TIMM17A (NP_006326.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:10 - 1:100 -
Validations
Western blot - TIMM17A Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using TIMM17A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Western blot - TIMM17A Polyclonal Antibody
Western blot analysis of extracts of various cells, using TIMM17A antibody at 1:1000 dilution ratio through one-step method.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.
Immunofluorescence - TIMM17A Polyclonal Antibody
Immunofluorescence analysis of MCF7 cells using TIMM17A antibody . Blue: DAPI for nuclear staining.
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Background
Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"