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Product Name
TIA1 antibody
- Documents
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Description
TIA1 Rabbit Polyclonal antibody. Positive IP detected in Jurkat cells. Positive WB detected in Jurkat cells, mouse spleen tissue, mouse thymus tissue, Raji cells. Observed molecular weight by Western-blot: 40 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Nucleolysin TIA 1 isoform p40 antibody; p40 TIA 1 antibody; RNA binding protein TIA 1 antibody; TIA 1 antibody; TIA1 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TIA1 recombinant protein (Accession Number: NM_001351518). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:116118(TIA1 antibody) at dilution of 1:200
IP Result of anti-TIA1 (IP:Catalog No:116118, 4ug; Detection:Catalog No:116118 1:500) with Jurkat cells lysate 3200ug.
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Background
TIA1, also named as p40-TIA-1, is involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). It possesses nucleolytic activity against cytotoxic lymphocyte target cells. TIA1 may be involved in apoptosis. Two isoforms of this protein exist - 41kDa and 42kDa.
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References
- Zhao W, Zhao J, Hou M. HuR and TIA1/TIAL1 are involved in regulation of alternative splicing of SIRT1 pre-mRNA. International journal of molecular sciences. 15(2):2946-58. 2014.
- Geng Z, Li P, Tan L, Song H. Targeted Knockdown of RNA-Binding Protein TIAR for Promoting Self-Renewal and Attenuating Differentiation of Mouse Embryonic Stem Cells. Stem cells international. 2015:657325. 2015.
- Hu S, Li J, Xu F. SAMHD1 Inhibits LINE-1 Retrotransposition by Promoting Stress Granule Formation. PLoS genetics. 11(7):e1005367. 2015.
- Wang Z, Tollervey J, Briese M, Turner D, Ule J. CLIP: construction of cDNA libraries for high-throughput sequencing from RNAs cross-linked to proteins in vivo. Methods (San Diego, Calif.). 48(3):287-93. 2009.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"