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Product Name
THTPA antibody
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Description
THTPA Rabbit Polyclonal antibody. Positive IP detected in K-562 cells. Positive WB detected in K-562 cells, A431 cells, HeLa cells, human brain tissue, mouse testis tissue, mouse uterus tissue, PC-3 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human stomach cancer tissue. Observed molecular weight by Western-blot: 26-35kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
thiamine triphosphatase antibody; THTP antibody; THTPA antibody; THTPASE antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of THTPA recombinant protein (Accession Number: NM_024328). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:116058(THTPA antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human stomach cancer using Catalog No:116058(THTPA antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using THTPA antibody Catalog No:116058 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-THTPA (IP:Catalog No:116058, 3ug; Detection:Catalog No:116058 1:800) with K-562 cells lysate 1720ug.
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Background
THTPA represents thiamine triphosphatase
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References
- Liu S, Miriyala S, Keaton MA. Metabolic effects of acute thiamine depletion are reversed by rapamycin in breast and leukemia cells. PloS one. 9(1):e85702. 2014.
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