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Product Name
THRSP antibody
- Documents
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Description
THRSP Rabbit Polyclonal antibody. Positive WB detected in mouse liver tissue, Transfected HEK-293 cells. Positive IF detected in MCF-7 cells. Positive IHC detected in human breast cancer tissue. Observed molecular weight by Western-blot: 20kd
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Tested applications
ELISA, IHC, IF, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
S14 antibody; Spot 14 protein antibody; SPOT14 antibody; THRSP antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of THRSP recombinant protein (Accession Number: NM_003251). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:116057(THRSP Antibody) at dilution of 1:1000
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:116057(THRSP Antibody) at dilution of 1:700
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:116057(THRSP Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of MCF-7 cells using Catalog No:116057(THRSP Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
THRSP (Thyroid hormone-inducible hepatic protein) is a protein that in humans is encoded by the THRSP gene. The protein encoded by this gene is similar to the gene product of S14, a rat gene whose expression is limited to liver and adipose tissue and is controlled by nutritional and hormonal factors. This gene has been shown to be expressed in liver and adipocytes, particularly in lipomatous modules. It is also found to be expressed in lipogenic breast cancers, which suggests a role in controlling tumor lipid metabolism (RefSeq). This antibody works well in IHC, WB and IF.
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