• Thanatin peptide (TFA removed)

Thanatin peptide (TFA removed)

Not For Human Use, Lab Use Only.

Cat.#: 309243


Special Price 378.5 USD

Availability: In Stock
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Product Information

  • Product Name
    Thanatin peptide (TFA removed)
  • Documents
  • Quantity/Unit
    1 Vial
  • Sequence
  • Three letter code
    H-Gly-Ser-Lys-Lys-Pro-Val-Pro-Ile-Ile-Tyr-Cys-Asn-Arg-Arg-Thr-Gly-Lys-Cys-Gln-Arg-Met-OH (disulfide bond: Cys11-Cys18)
  • Length (aa)
  • Peptide Purity (HPLC)
  • Molecular Formula
  • Molecular Weight
  • Source
  • Additional Information
    Thanatin is an inducible 21-residue cationic antimicrobial peptide that was first isolated from the hemolymph of the hemipteran insect Podisus maculiventris (spined soldier bug), through immune challenge. Thanatin is strongly cationic (pI of 10.48) and contains a distinct short eight-residue basic loop created through a disulfide bond formation between residues Cys11 and Cys18 at the C-terminus. Thanatin exerts bactericidal activity by disrupting outer membrane(OM) integrity. Thanatin damages the OM by promoting the release of divalent cations. Thanatin competitively replaces the divalent cations to bind to LPS, thereby disrupting the integrity of OM and leading to bacterial death.
  • Storage Guidelines
    Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides
  • References
    • Ma, B., Fang, C., Lu, L. et al. The antimicrobial peptide thanatin disrupts the bacterial outer membrane and inactivates the NDM-1 metallo-β-lactamase. Nat Commun 10, 3517 (2019).
    • Dash R, Bhattacharjya S. Thanatin: An Emerging Host Defense Antimicrobial Peptide with Multiple Modes of Action. Int J Mol Sci. 2021 Feb 3;22(4):1522. doi: 10.3390/ijms22041522. PMID: 33546369; PMCID: PMC7913509.
  • About TFA salt

    Trifluoroacetic acid (TFA) is a strong acid, which is commonly used to cleave synthesized peptides from solid-phase resins and is also used to improve HPLC performance in the peptide purification step. By default, custom peptides are delivered as lyophilized TFA salts, and can contain as much as 10-45% TFA.

    TFA in custom peptides can cause inexplicable discrepancies in subsequent assay data. For instance, TFA in nM concentrations has been shown to interfere with cellular assays, inhibiting cellular proliferation in some instances, and increasing cell viability in others . It has also been found to be an unintended allosteric modulator of the glycine receptor, GlyR.

    TFA Removal Service is recommended for:

    • Peptides that will be used in cellular assays
    • Peptides that will be used as APIs or in manufactured products
    • For hydrophilic peptides containing numerous basic residues

Peptide Property

  • Chemical Formula:C103H177N35O27S3
  • Sequence length:21
  • Extinction coefficient:1400 M-1cm-1
  • GRAVY:-0.9
  • Mw average:2433.91
  • Theoretical pI:10.87
  • Data Source:Peptide Property Calculator

GRAVY = grand average of hydropathy

X: Hydrophobic uncharged residues, like F I L M V W A and P

X: Basic residues, like R K H

X: Acidic residues, like D E

X: Polar uncharged residues, like G S T C N Q and Y

Peptide Services: NovoPro's peptide synthesis services include standard chemical peptide synthesis, peptide modification, peptide libraries, and recombinant peptide expression.

Standard Peptide Synthesis: NovoPro offers quality peptides at the most competitive prices in the industry, starting at $3.20 per amino acid. NovoPro provides PepBox – Automatic Quote Tool for online price calculation.

Peptide Modifications: NovoPro offers a wide range of peptide modification services including isotope labeling (2H, 15N, and 13C), multiple disulfide bonds, multiple phosphorylations, KLH, BSA, ovalbumin, amidation, acetylation, biotin, FITC, etc.