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Product Name
TH antibody
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Description
TH Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in mouse brain tissue. Positive IF detected in SH-SY5Y cells. Positive IHC detected in mouse brain tissue, rat brain tissue. Observed molecular weight by Western-blot: 50-55 kDa
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Tested applications
ELISA, WB, IF, IHC, IP
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
TYH antibody; Tyrosine 3 hydroxylase antibody; Tyrosine 3 monooxygenase antibody; tyrosine hydroxylase antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TH recombinant protein (Accession Number: NM_000360). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:116034(TH Antibody) at dilution of 1:600
IP Result of anti-TH (IP:Catalog No:116034, 4ug; Detection:Catalog No:116034 1:500) with mouse brain tissue lysate 3680ug.
Immunohistochemistry of paraffin-embedded mouse brain tissue slide using Catalog No:116034(TH Antibody) at dilution of 1:200 (under 40x lens).
Immunohistochemistry of paraffin-embedded rat brain tissue slide using Catalog No:116034(TH Antibody) at dilution of 1:200 (under 40x lens).
Immunofluorescent analysis of SH-SY5Y cells using Catalog No:116034(TH Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
TH(Tyrosine 3-monooxygenase) converts L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), the essential and rate-limiting step to formation of dopamine and other catecholamines. TH plays an important role in the physiology of adrenergic neurons and can be used as a marker for dopaminergic and noradrenergic neurons. This protein has 6 isoformsi produced by alternative splicing with the MW from 44 kDa to 58 kDa.
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