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Product Name
TFIP11 antibody
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Description
TFIP11 Mouse Monoclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human testis tissue, human prostate cancer tissue. Positive WB detected in HeLa cells, human brain tissue, human testis tissue. Observed molecular weight by Western-blot: 97 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human; other species not tested.
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Alternative names
bK445C9.6 antibody; FLJ22086 antibody; STIP antibody; STIP 1 antibody; TFIP11 antibody; TIP39 antibody
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Isotype
Mouse IgG2b
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Preparation
This antibody was obtained by immunization of TFIP11 recombinant protein (Accession Number: NM_001346859). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107620(TFIP11 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:107620(TFIP11 Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using Catalog No:107620(TFIP11 Antibody) at dilution of 1:50
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:107620(TFIP11 Antibody) at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
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Background
TFIP11, also named as Tuftelin-interacting protein 11 or Septin and tuftelin-interacting protein 1, is a 837 amino acid protein, which contains one G-patch domain and belongs to the TFP11/STIP family. TFIP11 may localizes in the nucleus and cytoplasm. TFIP11 is involved in pre-mRNA splicing, specifically in spliceosome disassembly during late-stage splicing events. TFIP11 may play a role in the differentiation of ameloblasts and odontoblasts or in the forming of the enamel extracellular matrix.
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