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Product Name
TFII I antibody
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Description
TFII I Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, HeLa cells, Jurkat cells. Positive FC detected in HeLa cells. Positive IHC detected in human ovary tumor tissue, human colon cancer tissue. Positive IF detected in Hela cells. Observed molecular weight by Western-blot: 135 kDa
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Tested applications
ELISA, WB, IF, FC, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
BAP 135 antibody; BAP135 antibody; BTK assOCiated protein 135 antibody; BTKAP1 antibody; DIWS antibody; FLJ38776 antibody; FLJ56355 antibody; GTF2I antibody; GTFII I antibody; IB291 antibody; SPIN antibody; SRF Phox1 interacting protein antibody; TFII I antibody; WBS antibody; WBSCR6 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TFII I recombinant protein (Accession Number: NM_001280800). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:116012(GTF2I antibody) at dilution of 1:800
Immunofluorescent analysis of Hela cells, using GTF2I antibody Catalog No:116012 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using Catalog No:116012(GTF2I Antibody) at dilution of 1:50 (under 40x lens)
1X10^6 HeLa cells were stained with 0.2ug GTF2I antibody (Catalog No:116012, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
The GTF2I gene encodes a transcription factor. It negatively regulates agonist-induced calcium entry into cells by interfering with expression of the cation channel TRPC3 at the plasma membrane [PMID: 17023658]. As a transcription factor and/or as a regulator of intracellular calcium levels, GTF2I may play a role in the molecular basis of anxiety[PMID: 22578324]. It interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation [PMID: 16738337].
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"