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Product Name
TFEB Polyclonal Antibody
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Description
Polyclonal antibody to TFEB
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
TFEB antibody; ALPHATFEB antibody; BHLHE35 antibody; TCFEB antibody; transcription factor EB antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant protein of human TFEB.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - TFEB Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using TFEB antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunofluorescence - TFEB Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using TFEB antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - TFEB Polyclonal Antibody
Immunofluorescence analysis of NIH/3T3 cells using TFEB antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Transcription factor that specifically recognizes and binds E-box sequences (5'-CANNTG-3'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Acts as a positive regulator of autophagy by promoting expression of genes involved in autophagy. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta.
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