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Product Name
TFEB antibody
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Description
TFEB Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, HeLa cells, human placenta tissue, mouse brain tissue, NIH/3T3 cells, rat brain tissue. Positive IP detected in NIH/3T3 cells. Positive IHC detected in human renal cell carcinoma tissue, human placenta tissue. Positive IF detected in Hela cells. Observed molecular weight by Western-blot: 65-70 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
AlphaTFEB antibody; bHLHe35 antibody; TCFEB antibody; TFEB antibody; transcription factor EB antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TFEB recombinant protein (Accession Number: XM_011514915). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:116009(TFEB antibody) at dilution of 1:1000
IP Result of anti-TFEB (IP:Catalog No:116009, 3ug; Detection:Catalog No:116009 1:1000) with NIH/3T3 cells lysate 3300ug.
Immunohistochemical of paraffin-embedded human renal cell carcinoma using Catalog No:116009(TFEB antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human renal cell carcinoma using Catalog No:116009(TFEB antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of Hela cells, using TFEB antibody Catalog No:116009 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
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Background
Transcription factor EB(TFEB) is one of the transcription factors that contain a basic HLH-ZIP domain(for DNA binding ) and HLH and ZIP domains( for loigomerization). TFEB specifically recognizes and binds to E-box sequences, which required dimerization with itself or another MiT/TFE family member. It plays a role in T-cell-dependent antibody responses to activated CD4+ T-cells and thymus-dependent humoral immunity once associated with TFE3. It also activates lysosomal gene by binding the CLEAR-box sequence in the regulatory region. It involves in the signal transduction processes required for normal vascularization of the placenta. This is a rabbit polyclonal antibody raised against the full length of human TFEB
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