-
Product Name
TFAM antibody
- Documents
-
Description
TFAM Rabbit Polyclonal antibody. Positive IHC detected in human liver cancer tissue, human heart tissue. Positive WB detected in Jurkat cells. Observed molecular weight by Western-blot: 25 kDa
-
Tested applications
ELISA, WB, IHC
-
Species reactivity
Human, Mouse; other species not tested.
-
Alternative names
MtTF1 antibody; mtTFA antibody; TCF 6 antibody; TCF6 antibody; TCF6L1 antibody; TCF6L2 antibody; TCF6L3 antibody; TFAM antibody; Transcription factor 6 antibody; Transcription factor 6 like 2 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_003201). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
-
Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:115996(TFAM antibody) at dilution of 1:600
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:115996(TFAM antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:115996(TFAM antibody) at dilution of 1:50 (under 40x lens)
-
Background
TFAM, also named as TCF6L2, MtTF1, TCF6, TCF6L1, TCF6L3 and mtTFA, is a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. It is involved in mitochondrial transcription regulation. TFAM is required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. It activates transcription by binding immediately upstream of transcriptional start sites. TFAM is able to unwind and bend DNA.
-
References
- Yu H, Wark L, Ji H. Dietary wolfberry upregulates carotenoid metabolic genes and enhances mitochondrial biogenesis in the retina of db/db diabetic mice. Molecular nutrition & food research. 57(7):1158-69. 2013.
- Lin D, He H, Ji H. Wolfberries potentiate mitophagy and enhance mitochondrial biogenesis leading to prevention of hepatic steatosis in obese mice: the role of AMP-activated protein kinase α2 subunit. Molecular nutrition & food research. 58(5):1005-15. 2014.
- Xing S, Yang X, Li W. Salidroside stimulates mitochondrial biogenesis and protects against H₂O₂-induced endothelial dysfunction. Oxidative medicine and cellular longevity. 2014:904834. 2014.
- Zhang X, Zuo X, Yang B. MicroRNA directly enhances mitochondrial translation during muscle differentiation. Cell. 158(3):607-19. 2014.
- Liu Y, Lan L, Huang K. Inhibition of Lon blocks cell proliferation, enhances chemosensitivity by promoting apoptosis and decreases cellular bioenergetics of bladder cancer: potential roles of Lon as a prognostic marker and therapeutic target in baldder cancer. Oncotarget. 5(22):11209-24. 2014.
- Geng J, Sun X, Wang P. Kinases Mst1 and Mst2 positively regulate phagocytic induction of reactive oxygen species and bactericidal activity. Nature immunology. 16(11):1142-52. 2015.
- Li H, Ruan Y, Zhang K. Mic60/Mitofilin determines MICOS assembly essential for mitochondrial dynamics and mtDNA nucleoid organization. Cell death and differentiation. 23(3):380-92. 2016.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"