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Product Name
TEX10 antibody
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Description
TEX10 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells. Positive IF detected in HeLa cells. Positive IHC detected in human brain tissue. Observed molecular weight by Western-blot: 95-100 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
bA208F1.2 antibody; FLJ20287 antibody; Nbla10363 antibody; testis expressed 10 antibody; TEX10 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TEX10 recombinant protein (Accession Number: NM_017746). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:115954(TEX10 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human brain using Catalog No:115954(TEX10 antibody) at dilution of 1:200 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:115954(TEX10 antibody) at dilution of 1:200 (under 40x lens)
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:115954(TEX10 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
TEX10 is a component of some MLL1/MLL complex, at least composed of the core components MLL, ASH2L, HCFC1/HCF1, WDR5 and RBBP5. PELP1–TEX10–WDR18 complex is a regulator of ribosome biogenesis and suggests that its SUMO-controlled distribution coordinates the rate of ribosome formation. And this complex is required for the maturation and nucleolar release of the large ribosomal subunit.(PMID:21326211)
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References
- Finkbeiner E, Haindl M, Muller S. The SUMO system controls nucleolar partitioning of a novel mammalian ribosome biogenesis complex. The EMBO journal. 30(6):1067-78. 2011.
- Castle CD, Cassimere EK, Denicourt C. LAS1L interacts with the mammalian Rix1 complex to regulate ribosome biogenesis. Molecular biology of the cell. 23(4):716-28. 2012.
- Fanis P, Gillemans N, Aghajanirefah A. Five friends of methylated chromatin target of protein-arginine-methyltransferase[prmt]-1 (chtop), a complex linking arginine methylation to desumoylation. Molecular & cellular proteomics : MCP. 11(11):1263-73. 2012.
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