-
Product Name
TEAD3 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to TEAD3
-
Tested applications
WB, IHC, IF
-
Species reactivity
Human, Mouse
-
Alternative names
TEAD3 antibody; DTEF-1 antibody; ETFR-1 antibody; TEAD-3 antibody; TEAD5 antibody; TEF-5 antibody; TEF5 antibody; TEA domain transcription factor 3 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 130-330 of human TEAD3 (NP_003205.2).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - TEAD3 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using TEAD3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - TEAD3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human colon using TEAD3 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - TEAD3 Polyclonal Antibody
Immunofluorescence analysis of A549 cells using TEAD3 antibody .
-
Background
Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds to multiple functional elements of the human chorionic somatomammotropin-B gene enhancer.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"