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Product Name
TDRKH specific antibody
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Description
TDRKH specific Rabbit Polyclonal antibody. Positive IHC detected in human gliomas tissue. Positive IF detected in Hela cells. Positive WB detected in mouse testis tissue. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 67kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
TDRD2 antibody; TDRKH antibody; tudor and KH domain containing antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TDRKH specific recombinant protein (Accession Number: BC032690). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:2000-1:20000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:115930(TDRKH antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:115930(TDRKH antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of Hela cells, using TDRKH antibody Catalog No:115930 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-TDRKH (IP:Catalog No:115930, 4ug; Detection:Catalog No:115930 1:7000) with mouse brain tissue lysate 4000ug.
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Background
Tudor and KH domain-containing protein(TDRKH)Tudor domains are protein modules that mediate protein-protein interactions, potentially by binding to methylated ligands. Both KH and tudor domains are involved in binding RNA or single-strand DNA. Tudor also is a germ cell-specific protein with multiple Tudor domains and is involved in germ plasm formation and germ cell specification. TDRKH is one Tudor protein with a single Tudor domain.
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References
- Saxe JP, Chen M, Zhao H, Lin H. Tdrkh is essential for spermatogenesis and participates in primary piRNA biogenesis in the germline. The EMBO journal. 32(13):1869-85. 2013.
- Chen C, Jin J, James DA. Mouse Piwi interactome identifies binding mechanism of Tdrkh Tudor domain to arginine methylated Miwi. Proceedings of the National Academy of Sciences of the United States of America. 106(48):20336-41. 2009.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"