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TDP1 antibody

Cat.#: 115924

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Product Information

  • Product Name
    TDP1 antibody
  • Documents
  • Description
    TDP1 Rabbit Polyclonal antibody. Positive WB detected in MCF7 cells, HeLa cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 68 kDa
  • Tested applications
    ELISA, WB, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    FLJ11090 antibody; TDP1 antibody; Tyr DNA phosphodiesterase 1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of TDP1 recombinant protein (Accession Number: BC006083). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:500-1:5000

  • Validations

    MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:115924(TDP1 antibody) at dilution of 1:1000

    MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:115924(TDP1 antibody) at dilution of 1:1000

    IP Result of anti-TDP1 (IP:Catalog No:115924, 4ug; Detection:Catalog No:115924 1:1000) with HeLa cells lysate 1200ug.

    IP Result of anti-TDP1 (IP:Catalog No:115924, 4ug; Detection:Catalog No:115924 1:1000) with HeLa cells lysate 1200ug.

  • Background
    Tyrosyl DNA phosphodiesterase 1 (TDP1) is an enzyme capable of hydrolyzing phosphodiester bonds between tyrosine and the 3-phosphate of DNA, which are typically generated in a transient manner by DNA topoisomerase I (topo I) and it appears to be more mobile and diffusible than topo I, and it has a different distribution in the nucleus (PMID:15494395). TDP1 is involed in protecting cells against oxidative DNA damage, and with the impaired ability of TDP1-deficient cells to remove 3-phosphoglycolate (PMID:21041670). Defects in TDP1 are the cause of spinocerebellar ataxia autosomal recessive with axonal neuropathy (SCAN1)(PMID:17948061).
  • References
    • Perego P, Cossa G, Tinelli S. Role of tyrosyl-DNA phosphodiesterase 1 and inter-players in regulation of tumor cell sensitivity to topoisomerase I inhibition. Biochemical pharmacology. 83(1):27-36. 2012.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"