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TDP-43 (human specific) antibody

Cat.#: 107618

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Product Information

  • Product Name
    TDP-43 (human specific) antibody
  • Documents
  • Description
    TDP-43 (human specific) Mouse Monoclonal antibody. Positive IHC detected in human brain(FTLD-U) tissue, human brain tissue, human gliomas tissue, human pancreas cancer tissue, human pancreas tissue. Positive FC detected in MCF-7 cells. Positive WB detected in K-562 cells, human brain tissue, human heart tissue, human placenta tissue. Positive IP detected in K-562 cells. Observed molecular weight by Western-blot: 43 kDa
  • Tested applications
    ELISA, IP, FC, WB, IHC
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    ALS10 antibody; TAR DNA binding protein antibody; TAR DNA binding protein 43 antibody; TARDBP antibody; TDP 43 antibody; TDP43 antibody
  • Isotype
    Mouse IgG1
  • Preparation
    This antibody was obtained by immunization of TDP-43 (human specific) recombinant protein (Accession Number: BC001487). Purification method: Caprylic acid/ammonium sulfate precipitation.
  • Clonality
    Monoclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:5000-1:50000

    IP: 1:500-1:5000

    IHC: N/A

  • Validations

    40X of FTLD-U case stained by Catalog No:115925 and Catalog No:107618, showing dystrophic neurites. (Figs were provided by Linda K. Kwong)

    40X of FTLD-U case stained by Catalog No:115925 and Catalog No:107618, showing dystrophic neurites. (Figs were provided by Linda K. Kwong)

    K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:107618(TDP-43 Antibody) at dilution of 1:10000

    K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:107618(TDP-43 Antibody) at dilution of 1:10000

    IP Result of anti-TDP-43 (IP:Catalog No:107618, 5ug; Detection:Catalog No:107618 1:1000) with K-562 cells lysate 1720ug.

    IP Result of anti-TDP-43 (IP:Catalog No:107618, 5ug; Detection:Catalog No:107618 1:1000) with K-562 cells lysate 1720ug.

    1X10^6 MCF-7 cells were stained with 0.2ug TDP-43 antibody (Catalog No:107618, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500.

    1X10^6 MCF-7 cells were stained with 0.2ug TDP-43 antibody (Catalog No:107618, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500.

  • Background
    Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). 60019-2-Ig is a mouse monoclonal antibody recognizing the cleavage product of 20-30 kDa in addition to the native and phosphorylated forms of TDP-43. Immunohistochemical analyses of TDP-43 using this antibody detect both normal diffuse nuclear staining and insoluble inclusions in pathologic tissues. The epitope of 60019-2-Ig has been determined to locate at residues 203-209 (TEDELRE) by Hiroshi Tsuji et al. (2012), which is involved in the formation of pathologic TDP-43 aggregation. Notably this antibody only recognizes human TDP-43 but not reacts with mouse or rat TDP-43.
  • References
    • Lee EB, Lee VM, Trojanowski JQ, Neumann M. TDP-43 immunoreactivity in anoxic, ischemic and neoplastic lesions of the central nervous system. Acta neuropathologica. 115(3):305-11. 2008.
    • Rohn TT. Caspase-cleaved TAR DNA-binding protein-43 is a major pathological finding in Alzheimer's disease. Brain research. 1228:189-98. 2008.
    • Deng HX, Zhai H, Bigio EH. FUS-immunoreactive inclusions are a common feature in sporadic and non-SOD1 familial amyotrophic lateral sclerosis. Annals of neurology. 67(6):739-48. 2010.
    • Deng HX, Chen W, Hong ST. Mutations in UBQLN2 cause dominant X-linked juvenile and adult-onset ALS and ALS/dementia. Nature. 477(7363):211-5. 2011.
    • Necchi D, Lomoio S, Scherini E. Dysfunction of the ubiquitin-proteasome system in the cerebellum of aging Ts65Dn mice. Experimental neurology. 232(2):114-8. 2011.
    • Tsuji H, Nonaka T, Yamashita M. Epitope mapping of antibodies against TDP-43 and detection of protease-resistant fragments of pathological TDP-43 in amyotrophic lateral sclerosis and frontotemporal lobar degeneration. Biochemical and biophysical research communications. 417(1):116-21. 2012.
    • Cohen TJ, Hwang AW, Unger T, Trojanowski JQ, Lee VM. Redox signalling directly regulates TDP-43 via cysteine oxidation and disulphide cross-linking. The EMBO journal. 31(5):1241-52. 2012.
    • Inamori Y, Higuchi I, Inoue T. Inclusion body myositis coexisting with hypertrophic cardiomyopathy: an autopsy study. Neuromuscular disorders : NMD. 22(8):747-54. 2012.

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