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TCTN2 antibody

Cat.#: 115920

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Product Information

  • Product Name
    TCTN2 antibody
  • Documents
  • Description
    TCTN2 Rabbit Polyclonal antibody. Positive IF detected in hTERT-RPE1 cells. Positive IHC detected in human liver tissue. Positive IP detected in RPE1 cells(siRNA).
  • Tested applications
    ELISA, IP, IF, IHC
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    C12orf38 antibody; FLJ12975 antibody; TCTN2 antibody; TECT2 antibody; Tectonic 2 antibody; tectonic family member 2 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of TCTN2 recombinant protein (Accession Number: NM_024809). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    IP: 1:200-1:2000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    IP result of Human RPE1 cell from Dr. Kevin Corbit. anti-TCTN2(Catalog No:115920).

    IP result of Human RPE1 cell from Dr. Kevin Corbit. anti-TCTN2(Catalog No:115920).

    IF result from Dr. Corbit, Kevin. anti-TCTN2(Catalog No:115920) mark the transition zone of Human hTERT-RPE1 cells.

    IF result from Dr. Corbit, Kevin. anti-TCTN2(Catalog No:115920) mark the transition zone of Human hTERT-RPE1 cells.

    Immunohistochemical of paraffin-embedded human liver using Catalog No:115920(TCTN2 antibody) at dilution of 1:100 (under 40x lens)

    Immunohistochemical of paraffin-embedded human liver using Catalog No:115920(TCTN2 antibody) at dilution of 1:100 (under 40x lens)

    Immunohistochemical of paraffin-embedded human liver using Catalog No:115920(TCTN2 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human liver using Catalog No:115920(TCTN2 antibody) at dilution of 1:100 (under 10x lens)

  • Background
    TCTN2 (tectonic-2 or TECT2) is a type I membrane protein that belongs to the tectonic family, which consists of TCTN1, TCTN2 and TCTN3. Studies in mice suggest that tectonic may be involved in Hedgehog (Hh) signaling, and essential for ciliogenesis. Tectonic is component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. It exists two spilcing isoforms. The first aberrant transcript introduces 104 base pair from intron 13 and would delete 196 original amino acid, introduce two novel amino acids, and prematurely truncate the 697aa protein at residue number 504. The second transcript lacks exon 14 and would delete 195 original amino acids, introduce four novel amino acids, and prematurely truncate the 697 aa protein at residue number 507. The molecular weight of two isoforms are about 50kDa. We detected the 50kDa and 90kDa protein by immunoprecipitaion, but 50kDa protein may be the splicing isoform or non-specific band.
  • References
    • Sang L, Miller JJ, Corbit KC. Mapping the NPHP-JBTS-MKS protein network reveals ciliopathy disease genes and pathways. Cell. 145(4):513-28. 2011.
    • Garcia-Gonzalo FR, Corbit KC, Sirerol-Piquer MS. A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition. Nature genetics. 43(8):776-84. 2011.
    • Wang WJ, Tay HG, Soni R. CEP162 is an axoneme-recognition protein promoting ciliary transition zone assembly at the cilia base. Nature cell biology. 15(6):591-601. 2013.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"