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Product Name
TCP1 Polyclonal Antibody
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Description
Polyclonal antibody to TCP1
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
TCP1 antibody; CCT-alpha antibody; CCT1 antibody; CCTa antibody; D6S230E antibody; TCP-1-alpha antibody; t-complex 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 307-556 of human TCP1 (NP_110379.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - TCP1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using TCP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry - TCP1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat kidney using TCP1 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - TCP1 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using TCP1 antibody . Blue: DAPI for nuclear staining.
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Background
Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis. The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance. As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. The TRiC complex plays a role in the folding of actin and tubulin (Probable).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"