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Product Name
TCIRG1 Polyclonal Antibody
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Description
Polyclonal antibody to TCIRG1
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
TCIRG1 antibody; ATP6N1C antibody; ATP6V0A3 antibody; Atp6i antibody; OC-116kDa antibody; OC116 antibody; OPTB1 antibody; Stv1 antibody; TIRC7 antibody; Vph1 antibody; a3 antibody; V-type proton ATPase 116 kDa subunit a isoform 3 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human TCIRG1 (NP_006010.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:200 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - TCIRG1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using TCIRG1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
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Background
Part of the proton channel of V-ATPases (By similarity). Seems to be directly involved in T-cell activation.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"