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Product Name
TCF4 antibody
- Documents
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Description
TCF4 Rabbit Polyclonal antibody. Positive WB detected in mouse lung tissue, A549 cells, PC-13 cells. Positive IP detected in mouse lung tissue. Positive IHC detected in human testis tissue. Observed molecular weight by Western-blot: 72 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
bHLHb19 antibody; E2 2 antibody; ITF 2 antibody; ITF2 antibody; PTHS antibody; SEF 2 antibody; SEF2 antibody; SEF2 1 antibody; SEF2 1A antibody; SEF2 1B antibody; SL3 3 enhancer factor 2 antibody; TCF 4 antibody; TCF4 antibody; transcription factor 4 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TCF4 recombinant protein (Accession Number: XM_006722536). Purification method: Antigen Affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse lung tissue were subjected to SDS PAGE followed by western blot with Catalog No:115905(TCF4 Antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human testis slide using Catalog No:115905(TCF4 Antibody) at dilution of 1:50
IP Result of anti-TCF4 (IP:Catalog No:115905, 4ug; Detection:Catalog No:115905 1:500) with mouse lung tissue lysate 4000ug.
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Background
Transcription factor 4 (TCF4), also known as SEF2, binds to the immunoglobulin enchancer Mu-E5/KE5-motif. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription By similarity. Preferentially binds to either 5'-ACANNTGT-3' or 5'-CCANNTGG-3'.TCF4 has several splicing isoforms that are expressed differentially in tissues and during cancer progression(15547706, 12796377). This antibody reacts with 68kd and 72kd TCF4 proteins.
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