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Product Name
TCF25 Polyclonal Antibody
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Description
Polyclonal antibody to TCF25
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Tested applications
WB
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Species reactivity
Human
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Alternative names
TCF25 antibody; FKSG26 antibody; Hulp1 antibody; NULP1 antibody; PRO2620 antibody; hKIAA1049 antibody; transcription factor 25 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human TCF25 (NP_055787.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - TCF25 Polyclonal Antibody
Western blot analysis of extracts of U-87MG cells, using TCF25 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
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Background
May play a role in cell death control. Acts as a transcriptional repressor. Has been shown to repress transcription of SRF in vitro and so may play a role in heart development.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"