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Product Name
TCF12/HEB antibody
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Description
TCF12/HEB Rabbit Polyclonal antibody. Positive WB detected in mouse lung tissue, Raji cells. Positive IHC detected in human cervical cancer tissue. Observed molecular weight by Western-blot: 73kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
bHLHb20 antibody; DNA binding protein HTF4 antibody; E box binding protein antibody; HEB antibody; HsT17266 antibody; HTF4 antibody; TCF 12 antibody; TCF12 antibody; transcription factor 12 antibody; Transcription factor HTF 4 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TCF12/HEB recombinant protein (Accession Number: NM_207037). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
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Validations
mouse lung tissue were subjected to SDS PAGE followed by western blot with Catalog No:115904(TCF12 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:115904(TCF12 antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:115904(TCF12 antibody) at dilution of 1:50 (under 10x lens)
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Background
The product of TCF12 gene (TCF12, also called HTF4 or HEB) is a member of helix-loop-helix (HLH) protein family [PMID:22130667]. TCF12 is a transcriptional regulator that involved in the initiation of neuronal differentiation. It 's also known as E proteins, and activates transcription by binding to the E box (5'-CANNTG-3'), because of their direct DNA (E-box) binding ability [PMID: 19204326]. TCF12 was shown to suppress E-cadherin expression during the early stages of renal tubular epithelial cell dedifferentiation [PMID:17202424].
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References
- Thorsen K, Schepeler T, Øster B. Tumor-specific usage of alternative transcription start sites in colorectal cancer identified by genome-wide exon array analysis. BMC genomics. 12:505. 2011.
- Bates JG, Salzman J, May D. Extensive gene-specific translational reprogramming in a model of B cell differentiation and Abl-dependent transformation. PloS one. 7(5):e37108. 2012.
- Labreche K, Simeonova I, Kamoun A. TCF12 is mutated in anaplastic oligodendroglioma. Nature communications. 6:7207. 2015.
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