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Product Name
TCEB3 Polyclonal Antibody
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Description
Polyclonal antibody to TCEB3
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
ELOA antibody; SIII antibody; SIII p110 antibody; TCEB3 antibody; TCEB3A antibody; SIIIp110 antibody; elongin-A antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 679-798 of human TCEB3 (NP_003189.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - TCEB3 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using TCEB3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 30s.
Immunohistochemistry - TCEB3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human kidney cancer using TCEB3 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - TCEB3 Polyclonal Antibody
Immunofluorescence analysis of MCF7 cells using TCEB3 antibody .
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Background
SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"