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Product Name
TBX21,T-bet antibody
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Description
TBX21,T-bet Rabbit Polyclonal antibody. Positive WB detected in mouse thymus tissue. Positive IP detected in mouse thymus tissue. Observed molecular weight by Western-blot: 60-62 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
T bet antibody; T box 21 antibody; T box protein 21 antibody; T PET antibody; TBET antibody; TBLYM antibody; TBX21 antibody; TBX21 antibody; T bet antibody; Transcription factor TBLYM antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TBX21,T-bet recombinant protein (Accession Number: NM_013351). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:1000
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Validations
mouse thymus tissue were subjected to SDS PAGE followed by western blot with Catalog No:115890(TBX21,T-bet antibody) at dilution of 1:300
IP Result of anti-TBX21,T-bet (IP:Catalog No:115890, 4ug; Detection:Catalog No:115890 1:300) with mouse thymus tissue lysate 2800ug.
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Background
TBX21 is a transcription factor that drives the Th1 immune response primarily through promoting expression of the IFN-γ gene. TBX21 initiates TH1 lineage development from naive TH precursor cells both by activating TH1 genetic programs and by repressing the opposing TH2 programs. It has also been shown to play an influential role in inflammatory processes such as inflammatory bowel disease, autoimmune diseases such as rheumatoid arthritis, and immune-mediated conditions like asthma.
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References
- Shen YM, Zhao Y, Zeng Y. Inhibition of Pim-1 kinase ameliorates dextran sodium sulfate-induced colitis in mice. Digestive diseases and sciences. 57(7):1822-31. 2012.
- Lu Y, Li L, Yan H, Su Q, Huang J, Fu C. Endothelial microparticles exert differential effects on functions of Th1 in patients with acute coronary syndrome. International journal of cardiology. 168(6):5396-404. 2013.
- Chen H, Fu T, Suh WK. CD4 T cells require ICOS-mediated PI3K signaling to increase T-Bet expression in the setting of anti-CTLA-4 therapy. Cancer immunology research. 2(2):167-76. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"