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TBP antibody

Cat.#: 115879

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Product Information

  • Product Name
    TBP antibody
  • Documents
  • Description
    TBP Rabbit Polyclonal antibody. Positive IP detected in COLO 320 cells. Positive WB detected in COLO 320 cells. Observed molecular weight by Western-blot: 43 kDa
  • Tested applications
    ELISA, WB, IP
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    GTF2D antibody; GTF2D1 antibody; SCA17 antibody; TATA binding factor antibody; TATA box binding protein antibody; TATA box factor antibody; TATA sequence binding protein antibody; TBP antibody; TF2D antibody; TFIID antibody
  • Immunogen
    Human TBP Recombinant protein (His tag)
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of TBP recombinant protein (Accession Number: NM_003194). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

  • Validations

    COLO 320 cells were subjected to SDS PAGE followed by western blot with Catalog No:117339(TBP antibody) at dilution of 1:1000

    COLO 320 cells were subjected to SDS PAGE followed by western blot with Catalog No:117339(TBP antibody) at dilution of 1:1000

    IP Result of anti-TBP (IP:Catalog No:117339, 4ug; Detection:Catalog No:117339 1:500) with COLO 320 cells lysate 400ug.

    IP Result of anti-TBP (IP:Catalog No:117339, 4ug; Detection:Catalog No:117339 1:500) with COLO 320 cells lysate 400ug.

  • Background
    The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This antibody recognize human TBP with MW 40 kDa and mouse/rat Tbp with MW 34 kDa.
  • References
    • Ma X, You X, Zeng Y. Mycoplasma fermentans MALP-2 induces heme oxygenase-1 expression via mitogen-activated protein kinases and Nrf2 pathways to modulate cyclooxygenase 2 expression in human monocytes. Clinical and vaccine immunology : CVI. 20(6):827-34. 2013.
    • Xu F, Wang F, Yang T, Sheng Y, Zhong T, Chen Y. Differential drug resistance acquisition to doxorubicin and paclitaxel in breast cancer cells. Cancer cell international. 14(1):538. 2014.
    • Han Y, Wang L, Yao QP. Nuclear envelope proteins Nesprin2 and LaminA regulate proliferation and apoptosis of vascular endothelial cells in response to shear stress. Biochimica et biophysica acta. 1853(5):1165-73. 2015.
    • Lee Y, Chun SK, Kim K. Sumoylation controls CLOCK-BMAL1-mediated clock resetting via CBP recruitment in nuclear transcriptional foci. Biochimica et biophysica acta. 1853(10 Pt A):2697-708. 2015.
    • Zeng Y, Liu H, Kang K. Hypoxia inducible factor-1 mediates expression of miR-322: potential role in proliferation and migration of pulmonary arterial smooth muscle cells. Scientific reports. 5:12098. 2015.
    • Lee Y, Jang AR, Francey LJ, Sehgal A, Hogenesch JB. KPNB1 mediates PER/CRY nuclear translocation and circadian clock function. eLife. 4:. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"