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Product Name
TBL2 antibody
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Description
TBL2 Rabbit Polyclonal antibody. Positive IHC detected in human pancreas cancer tissue, human heart tissue, human pancreas tissue. Positive IF detected in HepG2 cells. Positive WB detected in COLO 320 cells, HeLa cells, human placenta tissue, Jurkat cells, mouse pancreas tissue, mouse placenta tissue. Positive IP detected in COLO 320 cells. Observed molecular weight by Western-blot: 45-50kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DKFZp434N024 antibody; TBL2 antibody; transducin (beta) like 2 antibody; Transducin beta like protein 2 antibody; WBSCR13 antibody; WS betaTRP antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TBL2 recombinant protein (Accession Number: NM_012453). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
COLO 320 cells were subjected to SDS PAGE followed by western blot with Catalog No:115877(TBL2 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:115877(TBL2 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using TBL2 antibody Catalog No:109111 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-TBL2 (IP:Catalog No:115877, 4ug; Detection:Catalog No:115877 1:800) with COLO 320 cells lysate 2400ug.
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Background
TBL2 belongs to a member of the beta-transducin protein family. This gene is commonly deleted in Williams-Beuren syndrome, a developmental disorder with multi-system manifestations caused by haploinsufficiency for contiguous genes deleted in chromosome region 7q11.23. TBL2 encodes a protein with four putative WD40 repeats, which may be involved in some intracellular signaling pathway.
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References
- Tsukumo Y, Tsukahara S, Furuno A, Iemura S, Natsume T, Tomida A. TBL2 is a novel PERK-binding protein that modulates stress-signaling and cell survival during endoplasmic reticulum stress. PloS one. 9(11):e112761. 2014.
- Tsukumo Y, Tsukahara S, Furuno A, Iemura S, Natsume T, Tomida A. The endoplasmic reticulum-localized protein TBL2 interacts with the 60S ribosomal subunit. Biochemical and biophysical research communications. 462(4):383-8. 2015.
- Tsukumo Y, Tsukahara S, Furuno A, Iemura S, Natsume T, Tomida A. TBL2 Associates With ATF4 mRNA Via Its WD40 Domain and Regulates Its Translation During ER Stress. Journal of cellular biochemistry. 117(2):500-9. 2016.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"