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Product Name
TBC1D23 antibody
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Description
TBC1D23 Rabbit Polyclonal antibody. Positive IP detected in HepG2 cells. Positive WB detected in HepG2 cells, HeLa cells. Positive IHC detected in human liver tissue, human kidney tissue, human ovary tissue, human spleen tissue, human testis tissue. Observed molecular weight by Western-blot: 78 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DKFZp667G062 antibody; FLJ11046 antibody; NS4ATP1 antibody; TBC1 domain family member 23 antibody; TBC1 domain family antibody; member 23 antibody; TBC1D23 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TBC1D23 recombinant protein (Accession Number: BC010221). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:115866(TBC1D23 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human liver using Catalog No:115866(TBC1D23 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:115866(TBC1D23 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-TBC1D23 (IP:Catalog No:115866, 4ug; Detection:Catalog No:115866 1:500) with HepG2 cells lysate 2400ug.
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Background
TBC1D23, also named as NS4ATP1, is a gene down-requlated by Pneumocystis infection. Pneumocystis pneumonia is a common opportunistic disease in AIDS patients. (PMID: 20377877) Recently it has been found that TBC1D23 gene is the most frequently mutated in MSI-H cell lines and protein expressions of TBC1D23 in tumors with mutation were down regulated.(PMID: 20824714) This antibody is specific to TBC1D23.
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References
- Shin N, You KT, Lee H. Identification of frequently mutated genes with relevance to nonsense mediated mRNA decay in the high microsatellite instability cancers. International journal of cancer. 128(12):2872-80. 2011.
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