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Product Name
TAU antibody
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Description
TAU Rabbit Polyclonal antibody. Positive WB detected in SH-SY5Y cells, HeLa cells, HT-1080 cells, mouse brain tissue, rat brain tissue. Positive IHC detected in human brain tissue, human gliomas tissue, human lung cancer tissue. Observed molecular weight by Western-blot: 68 kDa, 81 kDa,100 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
DDPAC antibody; FLJ31424 antibody; FTDP 17 antibody; MAPT antibody; MAPTL antibody; MSTD antibody; MTBT1 antibody; MTBT2 antibody; Neurofibrillary tangle protein antibody; Paired helical filament tau antibody; PHF tau antibody; PPND antibody; TAU antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TAU recombinant protein (Accession Number: NM_016841). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with Catalog No:115857(TAU antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human brain tissue slide using Catalog No:115857(TAU Antibody) at dilution of 1:200 (under 10x lens)
Immunohistochemistry of paraffin-embedded human brain tissue slide using Catalog No:115857(TAU Antibody) at dilution of 1:200 (under 40x lens)
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Background
The microtubule-associated protein TAU (MAPT or TAU) is encoded by MAPT gene, which locates on human chromosome 17q21, binds to the tubulin subunit of microtubule and promotes its assembly and stability. Most TAU is expressed in neurons, and TAU isoform is expressed in the peripheral nervous system while the others are expressed in the central nervous system. TAU links axonal microtubules with C-terminus to neural plasma membrane components with its N-terminus, suggesting the participation in intracellular signal transduction and neuron's development and viability. Present polyclonal anti-MAPT antibody was produced by immunizing animals with N-terminal of MAPT and can detect approx 70-kDa and 100-kDa bands in brain tissues.
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References
- Rodríguez-Navarro JA, Gómez A, Rodal I. Parkin deletion causes cerebral and systemic amyloidosis in human mutated tau over-expressing mice. Human molecular genetics. 17(20):3128-43. 2008.
- Yang JY, Ha SA, Yang YS, Kim JW. p-Glycoprotein ABCB5 and YB-1 expression plays a role in increased heterogeneity of breast cancer cells: correlations with cell fusion and doxorubicin resistance. BMC cancer. 10:388. 2010.
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