Taromycin A peptide
Three letter code
Peptide Purity (HPLC)
~82% similar to AP2203: daptomycin. The authors used a transformation-associated recombination (TAR)-based genetic platform to directly clone, refactor, and heterologously express a silent biosynthetic pathway to yield a new antibiotic analog of daptomycin. The marine actinomycete Saccharomonospora sp. CNQ-490 TAR gene cluster was successfully expressed. Taromycin A differs from Daptomycin only in serveal places: 1) residue T11 is an D-Ala; 2) W is chlorinated at position 6; 3) Kny is also chlorinated at position 4; and 4) it carried an octa-2,4-dienoyl lipid side chain. Warning: the sequence is a close mimic of the natural counterpart. For example, kynurenine-13 (Kyn) is represented by Y here. Refer to daptomycin for more details. Active against MRSA 0325 (MIC 12 ug/ml), E. faecalis 613, E. faecalis 447 (MIC 6 ug/ml), E. faecalis 613D, E. faecium 447D (MIC 50 ug/ml), and VRE 5938 (100 ug/ml). The source of Taromycin A is the marine actinomycete Saccharomonospora sp. CNQ-490.
Ideally Taromycin A peptide should be stored in a freezer at or below -9C. Taromycin A peptide should be refrigerated after reconstitution. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides
- Yamanaka K, Reynolds KA, Kersten RD, et al. Direct cloning and refactoring of a silent lipopeptide biosynthetic gene cluster yields the antibiotic taromycin A. Proc Natl Acad Sci USA. 2014;111(5):1957-62.
About TFA salt
Trifluoroacetic acid (TFA) is a strong acid, which is commonly used to cleave synthesized peptides from solid-phase resins and is also used to improve HPLC performance in the peptide purification step. By default, custom peptides are delivered as lyophilized TFA salts, and can contain as much as 10-45% TFA.
TFA in custom peptides can cause inexplicable discrepancies in subsequent assay data. For instance, TFA in nM concentrations has been shown to interfere with cellular assays, inhibiting cellular proliferation in some instances, and increasing cell viability in others . It has also been found to be an unintended allosteric modulator of the glycine receptor, GlyR.
TFA Removal Service is recommended for:
- Peptides that will be used in cellular assays
- Peptides that will be used as APIs or in manufactured products
- For hydrophilic peptides containing numerous basic residues
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