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Product Name
TAPBP Polyclonal Antibody
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Description
Polyclonal antibody to TAPBP
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
TAPBP antibody; NGS17 antibody; TAPA antibody; TPN antibody; TPSN antibody; tapasin antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-260 of human TAPBP (NP_757345.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100 -
Validations
Western blot - TAPBP Polyclonal Antibody
Western blot analysis of extracts of HT-29 cells, using TAPBP antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - TAPBP Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach using TAPBP Antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - TAPBP Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat testis using TAPBP Antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - TAPBP Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human gastric cancer using TAPBP Antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - TAPBP Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse testis using TAPBP Antibody at dilution of 1:200 (40x lens).
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Background
Involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (peptide loading).
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"