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  • TAP2 Polyclonal Antibody
  • TAP2 Polyclonal Antibody

TAP2 Polyclonal Antibody

Cat.#: 166603

Special Price 174.2 USD

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Product Information

  • Product Name
    TAP2 Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to TAP2
  • Tested applications
    WB, IHC, IF
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    TAP2 antibody; ABC18 antibody; ABCB3 antibody; APT2 antibody; D6S217E antibody; PSF-2 antibody; PSF2 antibody; RING11 antibody; antigen peptide transporter 2 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 430-680 of human TAP2 (NP_000535.3).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IHC 1:50 - 1:200
    IF 1:50 - 1:200
  • Validations

    Western blot - TAP2 Polyclonal Antibody

    Western blot - TAP2 Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using TAP2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

    Immunofluorescence - TAP2 Polyclonal Antibody

    Immunofluorescence - TAP2 Polyclonal Antibody

    Immunofluorescence analysis of U2OS cells using TAP2 antibody . Blue: DAPI for nuclear staining.

  • Background
    Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin. Inhibited by the covalent attachment of herpes simplex virus ICP47 protein, which blocks the peptide-binding site of TAP. Inhibited by human cytomegalovirus US6 glycoprotein, which binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP1 and prevents the conformational rearrangement of TAP induced by peptide binding. Inhibited by human adenovirus E3-19K glycoprotein, which binds the TAP complex and acts as a tapasin inhibitor, preventing MHC class I/TAP association.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"