TAP2 Polyclonal Antibody
Polyclonal antibody to TAP2
WB, IHC, IF
Human, Mouse, Rat
TAP2 antibody; ABC18 antibody; ABCB3 antibody; APT2 antibody; D6S217E antibody; PSF-2 antibody; PSF2 antibody; RING11 antibody; antigen peptide transporter 2 antibody
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 430-680 of human TAP2 (NP_000535.3).
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Western blot - TAP2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using TAP2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence - TAP2 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using TAP2 antibody . Blue: DAPI for nuclear staining.
Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin. Inhibited by the covalent attachment of herpes simplex virus ICP47 protein, which blocks the peptide-binding site of TAP. Inhibited by human cytomegalovirus US6 glycoprotein, which binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP1 and prevents the conformational rearrangement of TAP induced by peptide binding. Inhibited by human adenovirus E3-19K glycoprotein, which binds the TAP complex and acts as a tapasin inhibitor, preventing MHC class I/TAP association.
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