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Product Name
Talin-1 antibody
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Description
Talin-1 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HepG2 cells, NIH/3T3 cells. Positive FC detected in HepG2 cells. Positive IHC detected in human colon cancer tissue, human liver cancer tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 270kd,230kd
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Tested applications
ELISA, WB, IHC, IF, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ILWEQ antibody; KIAA1027 antibody; talin 1 antibody; TLN antibody; TLN1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Talin-1 recombinant protein (Accession Number: NM_006289). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:115845(TLN1 antibody) at dilution of 1:500
1X10^6 HepG2 cells were stained with 0.2ug TLN1 antibody (Catalog No:115845, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:115845(TLN1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:115845(TLN1 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using TLN1 antibody Catalog No:115845 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
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Background
TLN1 represents talin 1
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