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Product Name
TAF9 Polyclonal Antibody
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Description
Polyclonal antibody to TAF9
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Tested applications
WB, IHC
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Species reactivity
Human, Rat
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Alternative names
TAF9 antibody; MGC:5067 antibody; STAF31/32 antibody; TAF2G antibody; TAFII-31 antibody; TAFII-32 antibody; TAFII31 antibody; TAFII32 antibody; TAFIID32 antibody; TATA-box binding protein associated factor 9 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human TAF9 (NP_057367.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - TAF9 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using TAF9 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry - TAF9 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat heart using TAF9 antibody at dilution of 1:100 (40x lens).
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Background
Essential for cell viability. TAF9 and TAF9B are involved in transcriptional activation as well as repression of distinct but overlapping sets of genes. May have a role in gene regulation associated with apoptosis. TAFs are components of the transcription factor IID (TFIID) complex, the TBP-free TAFII complex (TFTC), the PCAF histone acetylase complex and the STAGA transcription coactivator-HAT complex. TFIID or TFTC are essential for the regulation of RNA polymerase II-mediated transcription.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"