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Product Name
TAF5 Polyclonal Antibody
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Description
Polyclonal antibody to TAF5
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
TAF5 antibody; TAF100 antibody; TAF2D antibody; TAFII-100 antibody; TAFII100 antibody; TATA-box binding protein associated factor 5 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 656-800 of human TAF5 (NP_008882.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:100 -
Validations
Western blot - TAF5 Polyclonal Antibody
Western blot analysis of extracts of mouse brain, using TAF5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - TAF5 Polyclonal Antibody
Immunofluorescence analysis of A-549 cells using TAF5 antibody . Blue: DAPI for nuclear staining.
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Background
TAFs are components of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TAFs components-TIIFD are essential for mediating regulation of RNA polymerase transcription. TAF5/TAFII100 interacts strongly with the histone H4-related TAF6/TAFII80 and the histone H3-related TAF9/TAFII31, as well as a stable complex comprised of both TAF5/TAFII80 and TAF6/TAFII31. Apparently weaker interactions of TAF5/TAFII100 with TBP, TAF1/TAFII250, TAF11/TAFII28, and TAF12/TAFII20, but not TAF7/TAFII55, also have been observed.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"