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Product Name
TAF3 Polyclonal Antibody
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Description
Polyclonal antibody to TAF3
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat
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Alternative names
TAF140 antibody; TAFII140 antibody; TAFII-140 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant protein of human TAF3.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - TAF3 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using TAF3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3min.
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Background
Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Required in complex with TBPL2 for the differentiation of myoblasts into myocytes. The complex replaces TFIID at specific promoters at an early stage in the differentiation process.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"