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TAF12 antibody

Cat.#: 115978

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Product Information

  • Product Name
    TAF12 antibody
  • Documents
  • Description
    TAF12 Rabbit Polyclonal antibody. Positive IP detected in Y79 cells. Positive WB detected in Y79 cells. Observed molecular weight by Western-blot: 21 kDa
  • Tested applications
    ELISA, WB, IP
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    TAF12 antibody; TAF15 antibody; TAF2J antibody; TAFII 20/TAFII 15 antibody; TAFII20 antibody; TAFII20/TAFII15 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of TAF12 recombinant protein (Accession Number: NM_001135218). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:1000

  • Validations

    Y79 cells were subjected to SDS PAGE followed by western blot with Catalog No:115978(TAF12 antibody) at dilution of 1:300

    Y79 cells were subjected to SDS PAGE followed by western blot with Catalog No:115978(TAF12 antibody) at dilution of 1:300

    IP Result of anti-TAF12 (IP:Catalog No:115978, 4ug; Detection:Catalog No:115978 1:300) with Y79 cells lysate 2000ug.

    IP Result of anti-TAF12 (IP:Catalog No:115978, 4ug; Detection:Catalog No:115978 1:300) with Y79 cells lysate 2000ug.

  • Background
    TAF12, also named TAF15 or TAF2J, is a TBP-associated factor that is contained in Pol I- and Pol II-specific TBP-TAF complexes, it is also a component of the transcription factor IID (TFIID) complex, which is essential for mediating regulation of RNA polymerase transcription. TAF12 plays a key role in regulating eukaryotic gene expression by directly binding promoters and enhancer-bound transactivator proteins. Likewise, TAF12 recruits Gadd45a and the nucleotide excision repair complex to the promoter of rRNA genes leading to active DNA demethylation.
  • References
    • Guelman S, Kozuka K, Mao Y. The double-histone-acetyltransferase complex ATAC is essential for mammalian development. Molecular and cellular biology. 29(5):1176-88. 2009.
    • Kurihara N, Hiruma Y, Yamana K. Contributions of the measles virus nucleocapsid gene and the SQSTM1/p62(P392L) mutation to Paget's disease. Cell metabolism. 13(1):23-34. 2011.
    • Gegonne A, Tai X, Zhang J. The general transcription factor TAF7 is essential for embryonic development but not essential for the survival or differentiation of mature T cells. Molecular and cellular biology. 32(10):1984-97. 2012.
    • Teramachi J, Hiruma Y, Ishizuka S. Role of ATF7-TAF12 interactions in the vitamin D response hypersensitivity of osteoclast precursors in Paget's disease. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research. 28(6):1489-500. 2013.
    • Sun Q, Sammut B, Wang FM. TBK1 mediates critical effects of measles virus nucleocapsid protein (MVNP) on pagetic osteoclast formation. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research. 29(1):90-102. 2014.
    • Teramachi J, Zhou H, Subler MA. Increased IL-6 expression in osteoclasts is necessary but not sufficient for the development of Paget's disease of bone. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research. 29(6):1456-65. 2014.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"